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KMID : 0880220110490020315
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Journal of Microbiology
2011 Volume.49 No. 2 p.315 ~ p.315
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Development of Porphyromonas gingivalis-specific quantitative real-time PCR primers based on the nucleotide sequence of rpoB
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Park Soon-Nang
Park Jae-Yoon
Kook Joong-Ki
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Abstract
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Species-specific quantitative real-time PCR (qPCR) primers were developed for the detection of Porphyromonas gingivalis. These primers, Pg-F/Pg-R, were designed based on the nucleotide sequences of RNA polymerase β-subunit gene (rpoB). Species-specific amplicons were obtained from the tested P. gingivalis strains but not in any of the other strains (46 strains of 46 species). The qPCR primers could detect as little as 4 fg of P. gingivalis chromosomal DNA. These findings suggest that these qPCR primers are suitable for applications in epidemiological studies.
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KEYWORD
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Porphyromonas gingivalis, rpoB, species-specific, qPCR primers
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